The role of electronic microscopy in study and diagnosis of viral diseases

Electronic microscopy is essential for virus research and detection. To detect viruses or microorganisms, the study of appearance, dimentions, microstructure, parallel structure (cell, capsid, capsome and spikes), cell outer layer, pore and hair and comparison with the previous ones is vital through electronic microscopy. The advantage of this method is samples taken easily, quick classification, multifactoral causes and fast diagnosis.

Using XRD methods for the determination of asbetos which affect people's health in Vietnam environment

XRD (X Ray powder Diffraction) is an effective analysis method for crystallized materials. This method can identify and reflexes characteristics of asbestos in solid materials clearly based on 3 highest peaks of specific diffraction spectrum from asbestos containing samples. However, asbestos concentration lower than 1% may cause certain biases in analysis. Our study focused on the XRD method for qualitative determination of asbestos in solid material samples in Vietnam environment

Localization the antigens in cells by immunoelectronmicroscopic method

In the study, indirect immunogold labelling technique was used after embedding on verocells infected by measle virus and on PMK cells infected by rotavirus. According to black particles in immuno-electronic microscopic figure, rotavirus in PMK cells was localized and the formation of measle virus in vero cell was realized. 34. Trần Thanh Dương, Nguyễn Thu Vân, Hoàng Thủy Long: Molecula epidemiology of C hepatitis in hepatitis patients in Hanoi city. Vn J Pract Med 2004; 472(2):17-22:(CIMSI) Epidemiology; Hepatitis C; Fibrosis; Epidemiology, Molecular 323 patients with hepatitis and cirrhosis (244 males, 89 females admitted into the clinical Institute of Tropical diseases from Jan 2001 to August 2002 were studied on. Result showed that: in Hanoi, hepatitis patients were infected with C hepatitis virus of the types 1a, 1b, 6a. The incidence of various HCV genes was not different in deverse age groups, places, occupations, marital status of the patients, but the differences were noted according to the gender, the education level, the history of drug use and blood transfusion.

Immunoelectromicroscopic localization of surface antigens by GINS technique

GINS is Immunoelectromicroscopic technique combined indirect immunolabeling technique and negative staining technique in order to investigate surface antigens of particle specimens, such as virus, bacteria, debris of cell. This applicant investigation carry out on virus of JEV, HbsAg and salmonella. The results proved that samples not only have complete structure but also surface antigens with high specificity

To assess the duty filtration efficace of CT-2002 mouth cover, using electronic microscope

CT-2002 air filtering mouth mask Vietnam producted was studied on electronic microscope concerning the structure, the convenience and the efficacy of dust filtering. Results showed that its efficacy reached 85-87% in an air flow of 30 liters/minute but in the flow of 95 liter/minute, the efficacy reduced to 44-46%. The evaluation of efficacy was performed similarly to American and Japanese NIOSH method

The prosibility of Xray analysis and electronic microscope to determine abestos in working environment and lung tissues of pneumoconiosis patients

The study carries on 50g of lung tissues with pneumoconioses fixed in solution formol at faculty of morbid anatomy of 103 hospital. These tissues has been discovered both qualitative and quantitative by image and chart using electronic microscope X ray diffract method. On electronic microscope, there are amiang fibres with length approximately 1am and its diameter is very small, combining with intensity/energy chart of samples written on XRD machine, these showed that is Clinochyrysolite-Mg3Si205 (OH)4 with content 0,54%. It need to have a method to standardize electronic microscope X ray diffract method to discover, assess amiang (presence of any types on lung tissue) and quantitative samples of in labour environment

Immunoelectronmicroscopic localization of antigen in cells

Immunoelectronmicroscopic examination was applied largely in the diagnosis of microbioorganism. The technique of indirect immunogolden labelling was used after embedding on vero cell infected with measle virus and on TVP cell infected with rotavirus SA11. By black particles in immunoelectromicroscopic figure, the localization of rotavirus in TVP cell was determinated and the formation of measle virus in vero cell was identified.